FastQCFastQC Report
Thu 2 Jun 2016
DRR028214_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028214_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1801024
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19350.050.5686451
GTATCAA28750.045.3798681
ATCAACG27950.044.604143
TCAACGC27950.044.3448144
CAACGCA31400.039.4725345
AACGCAG33500.036.7817766
GTACATG58350.035.6508261
TATCAAC36000.035.2341772
TGACGCG1707.846029E-834.10905145
ACGCAGA37850.032.3630457
TACATGG57850.032.3254742
CGCAGAG38950.030.8908028
ACATGGG61800.029.4383743
ACGATAG3403.6379788E-1227.713602145
CATGGGG44100.024.9823654
CGACGAA4650.024.93995145
TAGATCG2101.5590073E-524.1605765
GTATACG2751.9752406E-723.7212941
GCAGAGT48550.023.5882879
GAGTACA44450.021.361321
Produced by FastQC (version 0.11.5)