FastQCFastQC Report
Thu 2 Jun 2016
DRR028214_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028214_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1801024
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29760.16523933051419637No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28630.15896512206389254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33500.074.132661
GTATCAA53850.051.916241
TCAACGC59950.045.589884
ATCAACG61550.044.5225523
CAACGCA64250.042.6515665
AACGCAG68050.040.4829146
TATCAAC68800.040.357722
GTACATG60450.036.397751
ACGCAGA79300.034.556917
CGCAGAG80400.033.9037788
TACATGG63550.033.7672
GTGGTAT17250.031.9928381
ACATGGG65650.031.2512953
GCAGAGT91400.029.5061849
TAGATCG2405.279071E-827.1861425
TGGTATC19100.026.5693532
CATGGGG48050.024.8946974
GAGTACA47250.023.2061651
CAGAGTA88400.022.8806449
CTCCGAC6800.022.397915145
Produced by FastQC (version 0.11.5)