FastQCFastQC Report
Thu 2 Jun 2016
DRR028213_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028213_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1954397
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC14100.053.9785145
GGTATCA20850.051.8001331
CGACGAA6350.047.943108145
TGACGCG2400.045.30339145
TCAACGC29850.042.7384724
GTATCAA32050.041.840261
ATCAACG30550.041.7591933
CAACGCA34300.037.405015
GTACATG63350.037.3010981
AACGCAG35950.035.688236
TACATGG64250.033.958152
ACGCAGA39800.032.0538527
TATCAAC41600.031.8866162
CGCAGAG42550.029.641518
ACATGGG69000.029.6244743
CATGGGG51000.025.1567044
GAGTACA45500.024.05561
GCAGAGT51550.023.0603479
GTGGTAT14300.021.7963141
AGTACAT49400.021.1293532
Produced by FastQC (version 0.11.5)