FastQCFastQC Report
Thu 2 Jun 2016
DRR028213_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028213_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1954397
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28020.14336902891275416No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26270.13441486044033019No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35100.075.6779251
GTATCAA54500.051.4027181
ATCAACG63300.043.0627143
TCAACGC63600.042.973574
CAACGCA66250.041.254635
AACGCAG69400.039.3821226
TATCAAC72500.037.899162
ACGCAGA79500.034.196487
CGCAGAG80350.033.473828
GTACATG58050.033.006311
GTGGTAT18500.032.1689871
TACATGG60400.029.5274892
GCAGAGT91500.028.9193829
ACATGGG63700.026.8593
TGGTATC19600.026.6320612
GTACCGT1902.1949204E-422.8936426
GAGTACA47850.022.5995391
CAGAGTA88800.022.2061469
CATGGGG48850.021.964154
AGTACAT49550.020.6302192
Produced by FastQC (version 0.11.5)