FastQCFastQC Report
Thu 2 Jun 2016
DRR028212_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028212_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1903878
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG24300.044.148923
GTATCAA27450.042.7796551
TCAACGC24650.042.3457834
GTACATG56600.040.342121
TGACGCG2350.040.099606145
CAACGCA27700.037.944855
GGTATCA17900.037.661231
TACATGG57800.036.8709342
AACGCAG31700.033.3855256
TATCAAC34800.031.869612
ACATGGG64250.031.5899893
ACGCAGA35450.029.2404757
CGCAGAG35550.028.5465138
TATAACG1555.5093373E-528.0597742
CATGGGG48250.025.5397244
GGATAGG17050.022.532848145
CCCGTAC2003.1080114E-421.7463263
GAGTACA43000.021.4091721
GCAGAGT46550.020.3993459
TATACGG3601.17956006E-720.1354872
Produced by FastQC (version 0.11.5)