FastQCFastQC Report
Thu 2 Jun 2016
DRR028212_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028212_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1903878
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22930.12043838943461714No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22760.11954547507770981No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29450.068.786621
GTATCAA50250.052.4512251
ATCAACG54950.047.496093
TCAACGC55700.046.596244
CAACGCA58250.044.9297835
AACGCAG61350.042.8958326
TATCAAC63950.040.9250952
ACGCAGA71400.036.5533647
CGCAGAG72500.035.8987668
GTACATG58400.035.6824071
TACATGG59750.031.9110362
GTGGTAT16200.030.9256741
GCAGAGT83850.030.6071769
ACATGGG60500.030.0774783
GAGTACA42900.029.280191
TGGTATC18850.025.3837432
AGTACAT47700.024.3178222
CTCCGAC7500.023.203468145
CATGGGG47050.022.804744
CAGAGTA79700.022.740759
Produced by FastQC (version 0.11.5)