FastQCFastQC Report
Thu 2 Jun 2016
DRR028211_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028211_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964004
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC25450.044.432374
ATCAACG25800.044.391523
GGTATCA18550.043.3752251
CAACGCA28300.039.957735
GTATCAA29500.039.8066561
GTACATG58350.037.7655141
AACGCAG30050.037.3895236
TACATGG59350.035.1750222
TATCAAC33150.034.7677272
ACATGGG62000.032.853173
ACGCAGA35500.031.649447
CGCAGAG37100.029.698358
CATGGGG47000.026.835774
TGACGCG3255.0931703E-1126.764587145
TCTCGGG20600.026.039173145
GAGTACA43150.025.0304181
GAGCGAC13000.023.976608145
AGTACAT45750.022.3403852
GCAGAGT46400.021.7149819
CCGACGG4952.1827873E-1120.501492145
Produced by FastQC (version 0.11.5)