FastQCFastQC Report
Thu 2 Jun 2016
DRR028211_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028211_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964004
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20910.10646617827662266No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26300.069.826091
GTATCAA46300.056.908691
TCAACGC53300.049.1023064
ATCAACG53550.048.873073
CAACGCA57200.046.1346555
AACGCAG59200.044.576056
TATCAAC60450.043.295612
ACGCAGA69000.038.034827
CGCAGAG71550.036.273998
CTCCGAC7200.032.229233145
GTACATG57850.031.4936731
GCAGAGT81300.031.2104059
TACATGG61150.028.572852
ACATGGG60450.028.4232653
CATGGGG44300.025.0385744
GTGGTAT15800.023.8890671
CAGAGTA78650.023.5973459
GAGTACA45550.023.4251251
TATAACG2704.4489116E-621.4812342
AGTACAT47750.020.8008062
Produced by FastQC (version 0.11.5)