FastQCFastQC Report
Thu 2 Jun 2016
DRR028210_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028210_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1673296
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17800.048.8689881
GACGACC16200.047.878536145
TCAACGC23800.043.5543174
GTATCAA26100.042.2158161
ATCAACG24750.041.8825343
GTACATG52100.038.1228181
CAACGCA27200.037.8435255
AACGCAG29200.035.4997526
TACATGG54350.034.6773532
ACGCAGA32350.031.8189747
ACATGGG58450.030.2605953
TATCAAC35250.029.612532
CGCAGAG35100.029.5325588
GAGTACA37400.026.3596361
CTTACGG6500.025.649954145
CATGGGG43650.024.0799664
GCAGAGT41700.023.4676659
GTCGTAC1600.002177676422.6528151
GTGGTAT11000.022.4056911
CCGACGC6300.021.861763145
Produced by FastQC (version 0.11.5)