FastQCFastQC Report
Thu 2 Jun 2016
DRR028210_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028210_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1673296
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24490.14635784702766277No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24230.14480402750021515No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28650.069.68981
GTATCAA45200.055.4168431
ATCAACG50800.049.092993
TCAACGC51050.048.710564
CAACGCA54150.045.9219635
AACGCAG57350.043.486036
TATCAAC60400.041.41142
ACGCAGA66800.037.117137
CGCAGAG67500.036.624818
GTACATG54750.035.274321
TACATGG55350.033.4010622
ACATGGG56900.031.980573
GCAGAGT78350.031.182849
GTGGTAT13350.028.8240971
CATGGGG42600.027.0590324
CTCCGAC8350.023.448002145
GAGTACA41400.022.7983881
TATAACG2552.7089081E-622.7450852
TGGTATC17150.021.9824942
TACACGC3304.696267E-821.9690275
Produced by FastQC (version 0.11.5)