FastQCFastQC Report
Thu 2 Jun 2016
DRR028209_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028209_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469863
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22850.046.952951
TCAACGC30650.045.4106944
ATCAACG32300.043.090953
GTATCAA36550.040.2620621
CAACGCA36000.038.8635255
GACGAAA9500.038.15345145
GTACATG78500.038.046521
AACGCAG38450.036.3871776
TACATGG80750.034.8318522
ACATGGG83400.032.50823
TATCAAC43950.032.1634832
TGACGCG3500.031.067806145
ACGCAGA45250.030.438447
CGCAGAG45200.029.9909738
CATGGGG65200.026.5728234
GAGTACA58450.024.3085421
GCCGACA8800.023.889261145
TAGATCG2653.7874688E-621.8842415
AGTACAT61100.021.711872
GCAGAGT62600.020.9600169
Produced by FastQC (version 0.11.5)