FastQCFastQC Report
Thu 2 Jun 2016
DRR028209_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028209_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469863
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32100.068.303381
GTATCAA53300.053.939671
TCAACGC62050.046.0340164
ATCAACG62900.045.527193
CAACGCA65450.043.531875
AACGCAG69050.041.157296
TATCAAC72450.039.3266872
GTACATG77000.036.017451
ACGCAGA79700.035.293757
CGCAGAG80150.034.8242388
TACATGG79150.032.3338172
ACATGGG81550.029.5147113
GCAGAGT93550.029.4485729
CATGGGG62900.025.0111394
GTGGTAT19450.024.6356851
TAGATCG2151.8676294E-523.6039125
GAGTACA57150.023.3744751
CAGAGTA94950.023.2878469
CTCCGAC9450.023.019346145
AGTACAT61850.020.7475472
Produced by FastQC (version 0.11.5)