FastQCFastQC Report
Thu 2 Jun 2016
DRR028208_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028208_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2370597
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG8050.048.62692145
GGTATCA22100.045.265331
ATCAACG30300.044.7381363
GTATCAA33200.044.323781
TCAACGC30650.043.990754
GTACATG79650.040.772851
TACATGG80750.038.3322452
CAACGCA36700.036.7388655
TATCAAC40400.034.9890482
ACATGGG87150.034.4359323
AACGCAG38800.034.3767626
ACGCAGA43650.029.5606947
TCTCGGG23100.029.498146145
CATGGGG67850.029.1669944
CGCAGAG46500.027.7489098
GAGTACA57650.026.5315151
CTTAGGG31650.026.339226145
AGTACAT60850.023.5875852
AGAGTAC50800.022.260755
GTACCGT2003.1076328E-421.747046
Produced by FastQC (version 0.11.5)