FastQCFastQC Report
Thu 2 Jun 2016
DRR028208_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028208_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2370597
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25440.10731473970480854No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23880.10073411887385329No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34350.066.789981
GTATCAA58900.058.057261
TCAACGC65700.050.979444
ATCAACG66450.050.5131453
CAACGCA69950.047.9856835
AACGCAG72900.046.0438776
TATCAAC78500.043.220982
ACGCAGA84700.039.1157077
CGCAGAG87300.037.7016228
GTACATG81900.034.7498631
GCAGAGT100100.032.446099
TACATGG82700.032.259732
ACATGGG84250.030.3754863
GTGGTAT19150.026.1596091
CATGGGG69000.026.0567954
GAGTACA60750.024.3800641
CTCCGAC10000.023.205807145
AGTACAT62200.022.6115232
TACGGGT3355.509537E-821.6408164
TGGTATC21850.021.5665342
Produced by FastQC (version 0.11.5)