FastQCFastQC Report
Thu 2 Jun 2016
DRR028207_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028207_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873040
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26800.045.7111971
TCAACGC25700.045.6933024
ATCAACG25750.045.604583
CGACGAC11350.042.790726145
CAACGCA27950.042.0149545
GGTATCA18350.041.4785841
AACGCAG29850.039.097796
GTACATG55350.036.0151941
TATCAAC34250.034.4983022
ACGCAGA34900.032.609567
TACATGG57900.031.5495112
ATCTACC17800.030.950258145
CGCAGAG37250.030.357718
ACATGGG62800.027.7027113
TCTCGGG18500.026.644518145
GCAGAGT44600.024.7046899
CATGGGG48050.022.7800254
GAGTACA43200.021.645951
TGACGCG2353.7158472E-521.592396145
AGTACAT42050.020.8588352
Produced by FastQC (version 0.11.5)