FastQCFastQC Report
Thu 2 Jun 2016
DRR028207_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028207_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873040
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23950.057.905721
GTATCAA38750.055.0895541
ATCAACG42050.049.8255923
TCAACGC42400.049.4143034
CAACGCA44800.046.767115
AACGCAG47800.044.135276
TATCAAC52150.040.036752
ACGCAGA55950.037.706277
CGCAGAG57150.036.787688
GTACATG54100.033.1507451
TACATGG55600.030.6417642
GCAGAGT69950.029.7450589
CTCCGAC7400.029.398565145
ACATGGG58500.028.2552833
CATGGGG44200.023.7829864
GAGTACA45250.023.748531
TCGTATC1952.61191E-422.31276145
AGTACAT44250.021.9539242
GTGGTAT14900.021.441741
CAGAGTA66750.020.961719
Produced by FastQC (version 0.11.5)