FastQCFastQC Report
Thu 2 Jun 2016
DRR028206_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028206_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2300165
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23150.045.4041331
TCAACGC34400.043.4097374
ATCAACG34600.043.368323
GTATCAA35550.043.2289431
CAACGCA39150.037.957755
GTACATG77750.037.666851
AACGCAG41600.035.896516
TACATGG79150.034.802552
CGACGAA7750.031.802084145
ACGATAG4350.031.66232145
ACATGGG82050.031.6288053
TATCAAC48900.030.982452
ACGCAGA47500.030.6747367
CGCAGAG49350.029.2310418
TGACGCG3151.036824E-925.313984145
GAGTACA56750.025.2916811
CATGGGG63750.024.5613354
GCAGAGT61350.022.3318969
AGTACAT60450.022.3046282
TAGATCG3004.566282E-721.7470135
Produced by FastQC (version 0.11.5)