FastQCFastQC Report
Thu 2 Jun 2016
DRR028206_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028206_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2300165
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26690.11603515399982176No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25520.1109485623857419No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37850.068.11111
GTATCAA58500.052.1373331
TCAACGC64800.045.7579654
ATCAACG65250.045.4423943
CAACGCA68300.043.5192645
AACGCAG72600.041.0415346
TATCAAC77150.038.3416632
GTACATG75100.034.9078141
ACGCAGA86400.034.0667467
CGCAGAG90650.032.4695788
TACATGG78450.032.1612552
GTGGTAT21050.030.3588961
ACATGGG80350.030.0453153
GCAGAGT99400.028.8090619
CATGGGG59450.024.9988694
TAGACCG1500.001495255924.1655755
TGGTATC24100.023.4652022
GAGTACA56200.022.6129441
ATGCCGC8100.021.487051145
CAGAGTA95850.021.1779699
Produced by FastQC (version 0.11.5)