FastQCFastQC Report
Thu 2 Jun 2016
DRR028205_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028205_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2422053
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC20350.061.622852145
TCAACGC32700.048.7680134
ATCAACG32850.048.7659843
GTATCAA34650.048.743061
CGACGAA10000.047.11655145
GGTATCA23100.044.245311
CAACGCA39900.039.9677665
AACGCAG42150.038.006236
TATCAAC45400.035.7645072
GTACATG80200.035.7012021
TACATGG81100.033.6961752
ACGCAGA47500.033.572927
CGCAGAG51150.031.17728
ACATGGG87000.030.994443
GAGTACA60800.025.9904021
CATGGGG66700.024.8868394
GCAGAGT64050.024.3320889
AGTACAT62150.023.559732
GAGCGTA1650.0026046321.9657559
AGAGTAC52950.020.945255
Produced by FastQC (version 0.11.5)