FastQCFastQC Report
Thu 2 Jun 2016
DRR028205_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028205_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2422053
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32800.064.6350561
GTATCAA53400.054.5207941
TCAACGC60400.048.0114864
ATCAACG61200.047.146963
CAACGCA64900.044.7941935
AACGCAG68100.042.9022456
TATCAAC69700.041.3981862
CTCCGAC8650.037.72628145
GTACATG82350.037.293731
ACGCAGA79250.036.7746737
CGCAGAG81100.035.7570088
TACATGG83200.035.5523152
ACATGGG86100.034.1015363
GCAGAGT92250.030.9636339
GTGGTAT19350.030.3922671
TCGTATC4600.028.376724145
CATGGGG67300.026.8229394
GAGTACA59750.026.3682251
AGTACAT64450.023.7350712
TGGTATC22150.023.2389032
Produced by FastQC (version 0.11.5)