FastQCFastQC Report
Thu 2 Jun 2016
DRR028204_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028204_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3183403
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC37550.049.226914
ATCAACG39050.047.335993
GTATCAA46250.043.101591
CAACGCA43200.042.788675
AACGCAG46750.039.384426
TGACGCG3900.039.03256145
GGTATCA29200.037.734021
GTACATG90700.034.6061251
TATCAAC55850.033.8758052
ACGCAGA53850.033.518617
TACATGG90100.032.744772
CGCAGAG54600.032.6598978
TAGACCG2101.4440957E-831.0667295
ACATGGG95900.029.7817333
GAGTACA67500.026.6330091
CATGGGG66800.026.2609984
TCTCGGG38350.024.383537145
GCAGAGT69450.024.21529
GGATAGG29000.023.99637145
AGTACAT79400.020.9980832
Produced by FastQC (version 0.11.5)