FastQCFastQC Report
Thu 2 Jun 2016
DRR028204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3183403
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39900.055.124541
GTATCAA65000.050.2544361
TCAACGC64000.048.595914
ATCAACG65650.047.8162573
CAACGCA68800.045.416255
AACGCAG74750.041.60726
TATCAAC82550.038.6424642
CTCCGAC10900.038.586895145
GTACATG97250.037.545131
TACATGG98900.035.3329542
ACGCAGA87000.035.16547
CGCAGAG89600.033.3358548
ACATGGG100750.033.3164183
GAGACGA10500.029.697336145
GCAGAGT105450.028.118959
CATGGGG72400.026.9361884
GAGTACA70500.026.1529031
TCGTATC5750.022.700897145
GTGGTAT25700.022.0311281
AGTACAT80900.021.2387542
Produced by FastQC (version 0.11.5)