FastQCFastQC Report
Thu 2 Jun 2016
DRR028203_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028203_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1617026
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC19300.040.187034
ATCAACG20500.038.1882173
GGTATCA16000.038.055621
CAACGCA21700.035.7423825
GTACATG46150.035.0261651
GTATCAA24050.034.359691
AACGCAG24150.032.7166526
TACATGG48650.031.8852922
GAGCGAC9700.031.386076145
ACATGGG49700.029.1697733
ACGCAGA27500.028.7311697
CGCAGAG27550.028.4159138
TATCAAC29750.027.2891832
TCTCGGG15800.027.067886145
CATGGGG33300.026.9921134
TGACGCG2156.138216E-726.971865145
GAGTACA33350.022.1699031
GCGATCA4607.2759576E-1222.061228145
GCAGAGT36000.021.343369
ATGGGGG23400.020.1352485
Produced by FastQC (version 0.11.5)