FastQCFastQC Report
Thu 2 Jun 2016
DRR028203_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028203_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1617026
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19310.11941675644052724No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18100.11193388356155065No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23850.063.0418551
GTATCAA37550.048.745851
ATCAACG41600.042.522973
TCAACGC42200.042.090174
CAACGCA43750.040.764695
AACGCAG46800.038.572756
TATCAAC50800.035.6793372
GTACATG43600.033.9852681
ACGCAGA54500.032.9899987
CTCCGAC5650.032.08281145
CGCAGAG56400.032.007178
TACATGG45550.031.5152
GTGGTAT14250.030.5832351
ACATGGG49250.028.704863
GCAGAGT66350.026.5517049
CAGAGTA65350.025.6267459
CATGGGG34500.024.7964784
TGGTATC15950.024.5455952
GAGACGA6000.024.169046145
CTACACT10950.021.1866824
Produced by FastQC (version 0.11.5)