FastQCFastQC Report
Thu 2 Jun 2016
DRR028202_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028202_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2126866
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACC18900.049.09228145
GGTATCA20250.047.2513161
TCAACGC29200.046.670244
ATCAACG29550.046.1174663
GTATCAA32950.042.6787151
CAACGCA32150.042.1624345
GTACATG61500.039.013771
AACGCAG36450.037.387416
TACATGG65900.035.0889432
TATCAAC40050.034.931712
ACATGGG70350.032.4572333
ACGCAGA40950.031.862787
CGCAGAG40850.031.2309868
CCGACGC7050.028.789488145
GCAGAGT47100.026.625049
GAGTACA45600.026.3881111
CATGGGG50700.025.7353234
AGTACAT50900.021.3618342
CATGGGA27900.020.0055984
GTATAGG9450.019.9437391
Produced by FastQC (version 0.11.5)