FastQCFastQC Report
Thu 2 Jun 2016
DRR028202_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028202_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2126866
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32050.062.523431
GTATCAA50350.054.0746271
TCAACGC53700.049.6823164
ATCAACG55200.048.3322533
CAACGCA57050.046.8920255
AACGCAG60600.044.623596
TATCAAC65550.041.6962432
ACGCAGA70250.037.7713977
CGCAGAG71650.036.8309978
GTACATG65250.034.605221
CTCCGAC8850.031.952944145
TACATGG67200.031.9338072
GCAGAGT83350.031.5739789
ACATGGG65400.031.4824773
GTGGTAT20300.024.6782761
CATGGGG48900.024.1661264
GAGTACA47950.023.1667211
TGGTATC22900.020.5781442
CAGAGTA82200.019.8444469
TACCGTA3001.1066964E-519.3329017
Produced by FastQC (version 0.11.5)