FastQCFastQC Report
Thu 2 Jun 2016
DRR028201_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028201_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2280920
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC27650.048.4979824
ATCAACG28700.046.7236633
CAACGCA31150.043.048775
GCCGACA6600.042.83194145
GTATCAA33700.042.5875171
AACGCAG32550.040.974526
GTACATG69400.040.524651
GGTATCA20200.038.3954241
TACATGG70000.037.8992082
GACGAAA9400.037.013523145
ACGCAGA37350.035.3205837
ACATGGG72650.034.720883
TATCAAC40700.034.1943132
CGCAGAG38850.033.58378
GAGTACA49450.028.2903331
CATGGGG49250.027.816514
TGACGCG2207.506733E-726.358116145
GCAGAGT48000.025.8227189
AGTACAT54600.023.4978282
CATGGGA30650.019.392354
Produced by FastQC (version 0.11.5)