FastQCFastQC Report
Thu 2 Jun 2016
DRR028201_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028201_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2280920
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33200.063.6407931
GTATCAA54300.052.2826581
TCAACGC59050.046.6537974
ATCAACG58950.046.609963
CAACGCA62100.044.3624275
AACGCAG66700.041.3029486
TATCAAC70000.039.5629462
CTCCGAC7600.036.255924145
ACGCAGA77000.035.40147
GTACATG67000.035.2199941
CGCAGAG78200.034.580038
TACATGG67250.033.2033352
ACATGGG69400.031.443463
GCAGAGT88750.030.1426419
GTGGTAT18600.028.496441
TAGATCG2603.7271093E-927.8836695
GAGTACA50050.026.5477491
GAGACGA8900.025.256939145
CATGGGG52050.024.6533454
CAGAGTA88350.024.1248199
Produced by FastQC (version 0.11.5)