FastQCFastQC Report
Thu 2 Jun 2016
DRR028200_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028200_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179065
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22300.050.3851361
TGACGCG5500.046.129734145
GTATCAA34700.043.2430651
TCAACGC32500.043.047664
ATCAACG33550.042.348613
CAACGCA36550.038.277675
AACGCAG38900.035.778926
GTACATG69050.035.3786931
TATCAAC44200.032.8007162
TACATGG70750.032.7868082
TCTCGGG19150.032.55407145
ACGCAGA43800.031.1142527
CGCAGAG44500.030.4619148
CTTAGGG24700.029.93497145
ACATGGG75750.029.5700073
CATGGGG55700.026.0285764
GAGTACA52600.024.1172561
GCAGAGT55650.023.9678049
GTCTCGC6550.023.2409341
GTATAGA14550.022.917671
Produced by FastQC (version 0.11.5)