FastQCFastQC Report
Thu 2 Jun 2016
DRR028200_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028200_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179065
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28060.12877082601941658No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27160.12464061420838755No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34250.069.952451
GTATCAA57800.057.0266151
TCAACGC65850.049.432424
ATCAACG66300.049.31563
CAACGCA68900.047.349415
AACGCAG71500.045.526226
TATCAAC75200.043.8646662
ACGCAGA83250.038.926437
CGCAGAG85950.037.6192638
GTACATG65800.032.7702751
GCAGAGT98400.032.491139
GTGGTAT17650.032.084811
TACATGG67950.030.4072192
ACATGGG68800.029.1885593
TGGTATC20600.024.9869022
ACCGCGT2651.3783938E-724.6216958
AGTACGG3251.4879333E-924.537525
GAGTACA50000.024.3943271
CATGGGG54450.023.4334414
CAGAGTA93850.022.4791569
Produced by FastQC (version 0.11.5)