FastQCFastQC Report
Thu 2 Jun 2016
DRR028199_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028199_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2980675
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAC18400.047.668373145
TCAACGC35500.046.7595064
ATCAACG35950.046.375833
GGTATCA25450.042.438621
GTATCAA41750.042.1902961
CAACGCA41600.039.9029435
GTACATG87200.037.823151
AACGCAG45050.036.8471156
TACATGG85950.036.433482
ACGCAGA47600.034.416317
CGCAGAG47150.033.668618
ACATGGG91200.032.6670383
TATCAAC53350.032.609162
TCTCGGG31200.030.43543145
ATCTACC28350.029.404078145
GAGTACA60600.027.392121
CATGGGG67250.026.192494
ACGATCC11350.024.268925145
GCAGAGT63550.023.7252379
AGTACAT70000.023.5066412
Produced by FastQC (version 0.11.5)