FastQCFastQC Report
Thu 2 Jun 2016
DRR028199_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028199_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2980675
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33250.056.7745251
GTATCAA53850.047.999431
TCAACGC53200.047.0143474
ATCAACG54500.045.8929063
CAACGCA57100.043.8032075
CTCCGAC9300.041.326866145
AACGCAG61300.040.920286
ACGCAGA70850.034.8929337
TATCAAC72500.034.6987952
GTACATG86000.034.107881
CGCAGAG72050.033.7080578
TACATGG85150.032.4386862
ACATGGG90100.029.8519063
GCAGAGT90300.026.0123869
GAGTACA63850.025.3580441
GAGACGA10300.025.34573145
CATGGGG63950.024.1469354
AGTACAT73650.021.1635572
CAGAGTA88750.021.1570139
CCACGAA9200.018.917463145
Produced by FastQC (version 0.11.5)