FastQCFastQC Report
Thu 2 Jun 2016
DRR028198_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028198_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3001611
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC39900.045.418514
ATCAACG40300.045.327453
GGTATCA29500.045.2128221
CGACGAA8200.042.431973145
GTATCAA46200.041.4216841
CAACGCA46750.038.9186635
GTACATG91950.038.8652081
TACATGG93250.036.069072
AACGCAG50650.036.0650836
ACATGGG95250.033.4091453
TATCAAC56700.033.111782
TGACGCG4400.032.949066145
ACGCAGA54200.032.9004337
CGCAGAG55400.031.6644128
CATGGGG64450.029.917454
ACGATAG5900.028.258013145
GAGTACA66450.026.180751
GCAGAGT70650.023.3931399
AGTACAT72800.022.4035552
GTATAGG14350.020.7108421
Produced by FastQC (version 0.11.5)