FastQCFastQC Report
Thu 2 Jun 2016
DRR028198_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028198_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3001611
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37200.065.758581
GTATCAA64150.056.8031651
TCAACGC70100.050.986064
ATCAACG70650.050.6917573
CAACGCA75300.047.368835
AACGCAG80050.044.7391936
TATCAAC86450.041.260752
ACGCAGA88950.040.099757
CGCAGAG91400.038.6282738
CTCCGAC10250.038.20331145
GTACATG90750.035.754141
TACATGG93550.032.781862
ACATGGG94100.031.8187393
GCAGAGT109900.031.0703249
GAGACGA10250.027.591278145
CATGGGG68600.026.5260264
GAGTACA66950.025.4790211
GTGGTAT21950.024.1409191
CAGAGTA105050.022.843079
AGTACAT72650.022.1541122
Produced by FastQC (version 0.11.5)