FastQCFastQC Report
Thu 2 Jun 2016
DRR028197_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028197_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2685519
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC21600.062.756535145
TCAACGC35250.050.5879484
ATCAACG35850.049.539093
GTATCAA41250.045.689931
CAACGCA40350.044.1939355
GGTATCA27250.043.892321
AACGCAG42100.041.6681636
CGACGAA10700.041.325405145
GTACATG84450.035.5362661
ACGCAGA49000.035.5047347
TATCAAC51650.035.2269022
TACATGG85050.033.0695652
CGCAGAG51750.032.9176338
ACATGGG88300.030.9493663
GAGTACA61950.027.497781
CATGGGG65800.025.7787024
GCAGAGT63800.025.5642419
AGTACAT70200.022.8205572
GTACGGA3254.002868E-822.3042556
AGAGTAC54550.020.4643085
Produced by FastQC (version 0.11.5)