FastQCFastQC Report
Thu 2 Jun 2016
DRR028197_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028197_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2685519
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28740.1070184199031919No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27600.1027734303871989No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36800.074.586791
GTATCAA64250.052.8920821
TCAACGC70300.048.0565454
ATCAACG71100.047.515833
CAACGCA73200.045.954595
AACGCAG77450.043.5264786
TATCAAC83700.040.536142
ACGCAGA88150.037.74967
CGCAGAG91100.036.3680348
GTACATG81550.034.1030161
CTCCGAC9400.032.402077145
TACATGG84750.031.4796642
GCAGAGT106200.030.7192089
ACATGGG87800.029.1475583
GAGTACA61600.026.9943431
GAGACGA8550.024.597063145
CATGGGG65100.024.3885024
AGTACAT70400.022.5524352
GTATACG3304.620233E-822.0041221
CAGAGTA103150.021.928429
Produced by FastQC (version 0.11.5)