FastQCFastQC Report
Thu 2 Jun 2016
DRR028196_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028196_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1654080
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18350.044.2434961
ATCAACG23700.042.514193
TCAACGC24050.042.1968884
GTATCAA25800.040.177581
CAACGCA27850.037.2201655
GTACATG51450.035.0817641
AACGCAG29400.034.764766
TACATGG54300.032.0389982
TATCAAC31450.032.0377242
ACATGGG56300.030.1283233
ACGCAGA34350.029.3329337
GTACCGT1753.7813606E-628.9952896
CGCAGAG35650.028.0599588
GTCTAGC3555.456968E-1226.5449851
TCTCGGG17150.025.782984145
CATGGGG42300.024.8482114
GTCTACG3402.5120244E-923.4520741
GGATAGG11800.023.343666145
GAGTACA38050.023.2419551
GTACGAC1650.002604026321.966133
Produced by FastQC (version 0.11.5)