FastQCFastQC Report
Thu 2 Jun 2016
DRR028196_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028196_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1654080
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25890.15652205455600696No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24930.1507182240278584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27750.068.030671
GTATCAA46600.054.067711
TCAACGC52300.046.991834
ACGGACC3550.046.978874145
ATCAACG52850.046.5027923
CAACGCA54150.045.3863835
AACGCAG56950.043.154926
TATCAAC59800.041.8256032
ACGCAGA65800.037.1302877
CGCAGAG66550.036.4939658
GTGGTAT17750.035.588961
GTACATG51800.035.043271
TACATGG54600.031.468792
GCAGAGT78550.030.7342349
ACATGGG55100.029.8674833
TGGTATC18000.028.5963232
GTATACG2156.0482853E-727.0175481
GAGTACA41150.025.7618711
CTCCGAC6600.025.26894145
CATGGGG42050.023.102724
Produced by FastQC (version 0.11.5)