FastQCFastQC Report
Thu 2 Jun 2016
DRR028195_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028195_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2028464
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC30650.048.95554
ATCAACG32200.046.3738373
GTATCAA33700.046.245581
GGTATCA21200.045.47551
CAACGCA33450.044.424185
GAGCGAC14750.042.755196145
AACGCAG35300.042.0959976
GTACATG69850.039.1233981
TATCAAC42000.035.725862
TACATGG73800.035.457862
ACGCAGA41100.035.449977
CGCAGAG44250.032.4349758
ACATGGG79900.032.1157573
CATGGGG61700.027.6085994
TCTCGGG21450.027.37281145
GCAGAGT54450.024.8946159
GAGTACA52450.023.6326431
AGTACAT53800.021.4228122
GTGGTAT15250.021.389681
TGACGCG2755.2229716E-621.087204145
Produced by FastQC (version 0.11.5)