FastQCFastQC Report
Thu 2 Jun 2016
DRR028195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2028464
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22240.1096396090835233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21150.10426608507718156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28900.073.0986251
GTATCAA50550.057.4450841
TCAACGC58400.049.1594734
ATCAACG58850.048.906763
CAACGCA61000.047.064155
AACGCAG65200.044.365996
ACGGACC4750.044.271637145
TATCAAC66850.043.3815272
ACGCAGA74100.038.5480887
CGCAGAG77250.036.882388
GTACATG69150.034.7496031
GTGGTAT15150.034.0219961
TACATGG72250.032.111342
GCAGAGT90250.031.2483639
ACATGGG76250.028.9991253
TGGTATC18650.026.4348222
CATGGGG57000.026.2009624
GAGTACA53000.023.148611
CAGAGTA86850.022.78869
TATAACG3157.2880357E-720.7146822
Produced by FastQC (version 0.11.5)