FastQCFastQC Report
Thu 2 Jun 2016
DRR028194_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028194_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891218
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACC17350.050.973125145
GGTATCA19500.045.353011
GTATCAA29300.045.2756161
TCAACGC29500.043.0028234
ATCAACG30200.042.4861373
CAACGCA34000.037.524485
GTACATG66700.037.495071
AACGCAG35000.036.2452356
TACATGG67800.034.5345462
CCGACGC7650.033.165577145
TATCAAC40150.032.318292
ACGCAGA40700.030.8129027
ACATGGG74550.030.7270153
CGCAGAG40800.030.3820368
CATGGGG52000.029.4144024
GAGTACA48700.025.304681
GTGGTAT12150.022.6719151
AGAGTAC45600.022.5737865
GCAGAGT51250.021.9239469
CAGAGTA49950.021.6237834
Produced by FastQC (version 0.11.5)