FastQCFastQC Report
Thu 2 Jun 2016
DRR028194_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028194_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891218
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26100.13800630070145273No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25080.1326129510188672No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30100.076.209931
GTATCAA52150.055.8188671
TCAACGC59650.048.372734
ATCAACG59750.048.2917673
CAACGCA62350.046.3942765
AACGCAG67200.043.261656
TATCAAC69150.042.253612
GTACATG66500.038.5339931
ACGCAGA75900.037.920737
CGCAGAG76700.037.5252088
GTGGTAT15300.036.533411
TACATGG66800.036.251082
ACATGGG70000.033.245663
GCAGAGT87850.031.9372259
TGGTATC17100.031.7991892
ACGGACC4800.031.722208145
CATGGGG53450.027.670084
GAGTACA49250.026.3838211
AGTACAT51800.023.7941442
GTATATA14900.021.4366721
Produced by FastQC (version 0.11.5)