FastQCFastQC Report
Thu 2 Jun 2016
DRR028193_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028193_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583329
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG23600.045.460243
TCAACGC23400.045.229214
GGTATCA17450.044.449861
GTATCAA26750.041.7329371
CAACGCA25750.041.1014985
GACGAAA6200.039.75294145
GTACATG53800.037.5927351
AACGCAG28500.037.1355636
TACATGG55250.034.9004752
TATCAAC33150.033.0198752
ACGCAGA32600.032.2427757
CGCAGAG32800.031.8251658
ACATGGG60200.030.7061753
CATGGGG44550.028.9637184
GAGTACA40800.025.2295911
GCAGAGT41700.023.8158749
AGTACAT42650.023.1154252
GCCGACA4850.022.41979145
CCCGTAC2104.319419E-420.7116153
CCTCGCG1800.00431138120.1362929
Produced by FastQC (version 0.11.5)