FastQCFastQC Report
Thu 2 Jun 2016
DRR028193_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028193_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583329
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26420.16686361457410304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24760.15637937535407992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26700.071.786881
GTATCAA42300.053.2076031
ATCAACG51050.043.7397273
TCAACGC51150.043.6542134
ACGGACC3100.042.10444145
CAACGCA53050.042.0907255
AACGCAG56250.040.340656
TATCAAC56600.039.5788422
GTGGTAT13350.035.34961
ACGCAGA65450.034.670157
GTACATG49050.034.6361241
CGCAGAG65850.034.3494578
TACATGG50050.032.7359852
ACATGGG52700.029.439083
TGGTATC16000.028.9988692
GCAGAGT76050.028.8844749
CATGGGG41000.024.9319534
CTAGTAC4303.6379788E-1223.6037333
CAGAGTA74550.023.5336239
CTACACG3655.693437E-921.8484634
Produced by FastQC (version 0.11.5)