FastQCFastQC Report
Thu 2 Jun 2016
DRR028192_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028192_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866627
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.055.4807971
TGACGCG4150.048.90859145
ATCAACG26300.048.7857173
TCAACGC26350.048.1429444
GTATCAA28800.046.8161471
CAACGCA30200.042.245545
AACGCAG31450.040.3359766
TATCAAC36600.035.848642
GTACATG58000.035.369881
ACGCAGA35700.035.127977
CGCAGAG37650.032.7309888
TACATGG60350.032.43112
CTTAGGG21950.029.061855145
TCTCGGG16300.028.90686145
GAGTACA43550.027.131551
ACATGGG68700.026.590043
GCAGAGT45950.024.610159
TATGTCG2451.9159706E-623.6700465
CATGGGG52450.023.495174
AGTACAT47000.023.2891852
Produced by FastQC (version 0.11.5)