FastQCFastQC Report
Thu 2 Jun 2016
DRR028192_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028192_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866627
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23480.12578838728894418No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22560.12085971112600429No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30400.071.631731
GTATCAA50050.056.4161261
TCAACGC57450.048.331584
ATCAACG57850.047.99743
CAACGCA60200.046.1237455
AACGCAG64050.043.464476
TATCAAC64800.043.4089242
ACGCAGA70550.039.0489047
CGCAGAG71400.038.5840348
TGGTATC17350.034.681742
GTACATG57750.034.4394571
GTGGTAT17950.034.3725851
TACATGG60450.033.3403972
GCAGAGT85800.031.9394049
ACATGGG63600.031.2331453
ACGGACC4600.028.3769145
TAGATCG2405.2787072E-827.1865165
CATGGGG48700.025.1582284
CTCCGAC5850.023.553095145
GAGTACA47400.022.6642381
Produced by FastQC (version 0.11.5)