FastQCFastQC Report
Thu 2 Jun 2016
DRR028191_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028191_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075301
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20550.046.9144671
TCAACGC29350.044.950064
ATCAACG29650.044.4952553
GTATCAA32850.042.36751
CGACGAC9600.042.284748145
CAACGCA32050.041.389495
AACGCAG33850.038.9744226
GTACATG61950.037.4434361
TATCAAC38850.034.8913352
TACATGG65000.033.7906272
ACGCAGA39400.032.380497
CGCAGAG40750.031.3077648
ACATGGG72150.030.040133
ATCTACC21750.026.662308145
TCTCGGG21650.024.776548145
ACGATCA9550.024.28922145
CATGGGG55550.024.271464
GCAGAGT51200.023.9267529
TTTCGCG2151.8693543E-523.6007929
GAGTACA47800.022.8989731
Produced by FastQC (version 0.11.5)