FastQCFastQC Report
Thu 2 Jun 2016
DRR028191_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028191_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075301
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29400.060.5013731
GTATCAA44950.050.5546571
TCAACGC50700.044.3273744
ATCAACG51250.043.993123
CAACGCA52750.042.742135
ACGGACC5300.041.047737145
AACGCAG55950.040.297546
TATCAAC58850.038.806392
GTACATG63750.034.8487931
ACGCAGA64500.034.5061847
CGCAGAG66050.033.367148
TACATGG65950.032.1001632
ACATGGG68000.029.9581933
GTGGTAT16850.029.7300511
CTCCGAC7000.026.935131145
GCAGAGT79300.026.8777189
CATGGGG52100.026.2991874
GAGTACA49600.022.3952661
TGGTATC20550.022.2263372
CAGAGTA75650.021.7538019
Produced by FastQC (version 0.11.5)