FastQCFastQC Report
Thu 2 Jun 2016
DRR028190_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028190_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2564129
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38600.050.8920631
TCAACGC38200.048.38884
ATCAACG38600.047.699573
GGTATCA25550.046.5287061
CAACGCA45800.040.359225
GTACATG85500.039.9321631
TACATGG86900.037.9541852
AACGCAG48900.037.94896
TATCAAC51750.037.1196252
ACGCAGA51400.035.1159367
ACGATAG3750.034.79439145
CGACGAA6900.034.668324145
ACATGGG95350.033.754393
CGCAGAG53500.032.3826338
CATGGGG68350.029.1650144
GAGTACA65150.027.9271971
AGTACAT64400.026.7891622
GCAGAGT66950.024.686099
TGACGCG2903.2953176E-722.496376145
ATACCGT4851.6370905E-1120.9244656
Produced by FastQC (version 0.11.5)