FastQCFastQC Report
Thu 2 Jun 2016
DRR028190_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028190_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2564129
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27680.10795088702635475No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27030.1054159131619353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40150.069.808821
GTATCAA64800.055.5795751
ACGGACC6800.047.98835145
TCAACGC75300.047.368784
ATCAACG75900.046.9943243
CAACGCA80150.044.6833275
AACGCAG85950.041.58376
TATCAAC88400.040.185962
ACGCAGA96250.036.8324137
GTACATG86350.036.66341
CGCAGAG97000.036.398158
TACATGG87350.034.5271642
ACATGGG92100.031.6437873
GCAGAGT111450.030.5080789
CATGGGG65100.028.7316654
GTGGTAT21700.026.7694111
GAGTACA63800.025.493881
AGTACAT65600.024.3136042
TGGTATC23350.023.5970252
CAGAGTA106950.022.9117349
Produced by FastQC (version 0.11.5)